Table S1.
PCR and sequencing primers
| cDNA and RNA templates | Sequence, 5′→ 3′ |
| Cloning Mecp2 target plasmids | |
| mMecp2 E1 Fwd EcoRI | gcgcgaattccaccatggccgccgctgccgccac |
| mMecp2 E1 Rev KpnI no stop codon | gcgcgggtaccgctaactctctcggtcacgggc |
| mMecp2 W104X mut primer Fwd | caccttgcctgaaggttagacacgaaagcttaaac |
| mMecp2 W104X mut primer Rev | gtttaagctttcgtgtctaaccttcaggcaaggtg |
| mMecp2 R106Q mut primer Fwd | cctgaaggttggacacaaaagcttaaacaaagg |
| mMecp2 R106Q mut primer Rev | cctttgtttaagcttttgtgtccaaccttcagg |
| mMecp2 R306H mut primer Fwd | cccatcaagaagcacaagacccgggag |
| mMecp2 R306H mut primer Rev | ctcccgggtcttgtgcttcttgatggg |
| Cloning Editase plasmids (pGM1090, pGM1091) | |
| Editase EcoRI Kozak HA Fwd | gaattcgccaccatggtgtacccctatgacgtgcctgactacgccagcggctatccatacgatgtccccgattatgctt |
| Editase HA BspEI Rev | ccggaagcataatcggggacatcgtatggatagccgcaggcgtagtcaggcacgtcataggggtaccatggtggcg |
| EGFP F2 | caccatcttcttcaaggacgac |
| SV40 3xNLS Rev | gacaatccggaggtggatcctacctttctctt |
| hDD E488Q Fwd | aatagagtctggtcaggggacgattcc |
| hDD E488Q Rev | ggaatcgtcccctgaccagactctatt |
| Guide RNA sequences | |
| mMecp2 W104X 2xBoxB Guide Fwd | caccgtcctttgtttggccctgaaaaagggccctttcgtgtccaaccttcaggcaaggggccctgaaaaagggccggtcatcatac |
| mMecp2 W104X 2xBoxB Guide Rev | aaaagtatgatgaccggccctttttcagggccccttgcctgaaggttggacacgaaagggccctttttcagggccaaacaaaggac |
| mMecp2 R106Q 2xBoxB Guide Fwd | caccgcagacttcctggccctgaaaaagggcctttaagctttcgtgtccaaccttcaggcaggccctgaaaaagggcctggggtcatc |
| mMecp2 R106Q 2xBoxB Guide Rev | aaaagatgaccccaggccctttttcagggcctgcctgaaggttggacacgaaagcttaaaggccctttttcagggccaggaagtctgc |
| mMecp2 R306H 2xBoxB Guide Fwd | Caccgatgctgaccgtggccctgaaaaagggccccgggtcttgcgcttcttgatgggagcaggccctgaaaaagggcctctcatgcaca |
| mMecp2 R306H 2xBoxB Guide Rev | Aaaatgtgcatgagaggccctttttcagggcctgctccccatcaagaagcgcaagacccggggccctttttcagggccacggtcagcatc |
| mMecp2 R106Q Off-target mismatch 2xBoxB Guide Fwd | Caccgcagacttcctggccctgaaaaagggcctttaagctttccgggtccaaccttcaggcaggccctgaaaaagggcctggggtcatc |
| mMecp2 R106Q Off-target mismatch 2xBoxB Guide Rev | aaaagatgaccccaggccctttttcagggcctgcctgaaggttggacccgaaagcttaaaggccctttttcagggccaggaagtctgc |
| Amplification of Mecp2-eGFP cDNA | |
| CMV-mMecp2 ATG Fwd | tcaagcttcgaattccaccatggcc |
| GFP-N3 Linker Rev | ccttgctcaccatggtggcga |
| Amplification of endogenous Mecp2 cDNA | |
| mMecp2-14 mMecp2 ATG Fwd | aacccgtccggaaaatggcc |
| mMecp2-3′UTR+92 Rev | ggaagctttgtcagagccctacccataag |
| Sequencing primers for Mecp2 RT-PCR | |
| mMecp2 554 Rev | ctcctggaggggctccctctc |
| mMecp2 914 Rev | gaccgtatggaagactccttca |
| mMecp2 1122 Rev | actgctgctgcgcccctt |
| Cloning of plasmids pGM1257 and pGM1258 | |
| hU6 AflII Fwd | gtgtcttaaggagggcctatttcccatgatt |
| hU6 MfeI Fwd | gtgtcaattggagggcctatttcccatgatt |
| hU6 NheI Fwd | gtgtgctagcgagggcctatttcccatgatt |
| hU6 SacI Fwd | gtgtgagctcgagggcctatttcccatgatt |
| hU6 SpeI Fwd | gtgtactagtgagggcctatttcccatg |
| hU6 (m) NdeI Fwd | gtgtcatatgcttaccgtaacttgaaag |
| R106QgV2 AflII Rev | atatcttaagaaaaaagatgaccccaggccct |
| R106QgV2 ApaI Rev | cacagggcccaaaaaagatgaccccaggccct |
| R106QgV2 MfeI Rev | atatcaattgaaaaaagatgaccccaggccct |
| R106QgV2 SacI Rev | atatgagctcaaaaaagatgaccccaggccct |
| R106QgV2 SpeI Rev | atatactagtaaaaaagatgaccccaggccct |
| R106QgV2 (+2)ApaI Rev | gcgcgggcccttcgaagctagcaaaaaagatgaccccaggccct |
| Cloning of Editase into plasmid pGM1257 | |
| 2xHA Editase Fwd NcoI Kozak | attcgccaccatggtgtacccctatgacgtg |
| Editase EcoRI Rev | gcgcgaattctcaatggtgatggtgatggt |