Table 1.

Kinetic parameters of reductive and re-oxidative half-reactions calculated for hFMO3 wild type and N61S variant.

	Reduction		Re-oxidation	Intermediate
	K d,NADPH	k red	k ox	k formation	k decay
	(μM)	(s −1 )	(s −1 )	(s −1 )	(s −1 )
Wild type	0.3	1.33	R 1 = 0.79 R 2 = 0.0009	1.580	0.001
N61S	51.8	ND	0.70	0.332	0.048
		2.42#

ND: not detected.

^#10-fold higher concentration of NADPH.