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. 2017 Nov 7;7:14763. doi: 10.1038/s41598-017-15344-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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dG9a regulates the expression of gustatory receptor genes. Quantification of mRNA levels by RT-qPCR analyses of Gr5a, Gr61a, Gr64a, Gr64b/c, Gr64d/e and Gr64f in Canton S and dG9a ^RG5 adult flies after 0 h, 6 h, 12 h and 24 h starvation. According to Flybase (http://Flybase.org), Gr64b and Gr64c are translated from the same transcript shown as Gr64b/Gr64c. Gr64d and Gr64e are also translated from the same transcript shown as Gr64d/Gr64e. Results were normalized to β-tubulin and are displayed as relative values for that of 0 h starved Canton S. n = 3. Error bars represent standard errors. *There is significant difference between Canton S and dG9a ^RG5 in each time point evaluated by unpaired two-tailed Student t-tests. *P < 0.05. There was also significant difference in Gr64a, Gr64b/c, Gr64d/e between Canton S and dG9a ^RG5 when a two-way factorial analysis of variance was applied (P < 0.05).