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. 2017 Nov 7;7:14763. doi: 10.1038/s41598-017-15344-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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dG9a depletion increases the locomotion activity under starvation. (A) The beam crossing activity of Canton S and the dG9a ^RG5 under starvation conditions. Gray and white background in the graph represents 12 h dark periods and 12 h light periods, respectively. (B) The beam crossing activity of Canton S and dG9a ^RG5 in the presence of 1 M sucrose. (C,D) Averaged total crossing in 30 h after start point (C) and during 2^nd light/dark cycle (D). 0 h starved Canton S: n = 31. 0 h starved dG9a ^RG5: n = 29. Canton S on 1 M sucrose: n = 24. dG9a ^RG5 on 1 M sucrose: n = 20. *P < 0.05. (E) Quantification of mRNA levels by RT-qPCR analyses of dG9a in wild type adult flies after 0 h, 6 h, 12 h and 24 h starvation. Results were normalized to β-tubulin and are displayed as relative values for that of 0 h starvation (n = 3). Error bars represent standard errors. *P < 0.05.