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. 2017 Nov 7;7:14763. doi: 10.1038/s41598-017-15344-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Refeeding after fasting restores the hyperactivity and increased sensitivity to sucrose as well as dG9a expression. (A) The beam crossing activity of Canton S under 24 h starvation conditions followed by 1 M sucrose refeeding. Gray and white background in the graph represents 12 h dark periods and 12 h light periods, respectively. (B) (left) Averaged total crossing in 24 h under 1 M sucrose feeding conditions. n = 24. (right) Averaged total crossing in 24 h under starvation conditions and refed conditions calculated from the data shown in Fig. 6A. n = 17. *P < 0.05. (C) Sucrose concentration-PER curves in Canton S under different starvation conditions and refed conditions (n = 3). (D) Quantification of mRNA levels by RT-qPCR analyses of dG9a in wild type adult flies after 0 h, 24 h starvation and after 24 h starvation followed by 24 h 1 M sucrose refeeding. Results were normalized to β-tubulin and are displayed as relative values for that of 0 h starvation (n = 3). Error bars represent standard errors. *P < 0.05.