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. 2017 Nov 7;7:14778. doi: 10.1038/s41598-017-15122-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Over all structural changes of microbiota community during quinoline-degrading process based 16S rRNA gene V1-V3 454 pyrosequencing. Comparison of relative abundances of the major phylotypes (relative abundances above 1%) found in the DNA and RNA data. (a) At phylum level (b) at genera level. (a) The left part represents the phylotypes from the DNA, and the right part represents the phylotypes from the RNA-based data. The colors correspond to the major phylogenetic groups of the phylotypes. The different parts of the stacked bars represent the phylotypes identified. (b) The different stars above the columns mean significant differences as assessed by Tukey’s test (*P < 0.05, ***P < 0.001). (c) Bray-curtis PCoA of the quinoline-degrading microbiota at time H0, H25, H80 and H144 based on pyrosequencing OTU (97% identity) data. The values in parenthesis indicate the percentage of community variation explained by the axes. (d) Clustering of microbiota based on mahalanobis distances between different groups calculated with multivariate analysis of variance test, ***P < 0.001.