Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 7;7:14641. doi: 10.1038/s41598-017-15269-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mammalian cell lines transfected with CHIKV-D-Luc-SGR. (a) Schematic of the CHIKV genome and the corresponding dual luciferase replicon (CHIKV-D-luc-SGR). Renilla luciferase (Rluc) is present in the hypervariable region of nsP3, indicating both input translation and early replication levels. Firefly luciferase (Fluc) replaces the structural genes and is only expressed from a subgenomic RNA, thus indicating that RNA replication has occurred. Note that other SGRs used in this study have either no insertion into nsP3 (termed nsP3/SG-FLuc), or insertion of mCherry in place of Rluc and Gaussia luciferase in place of Fluc (nsP3-mCherry/SG-GLuc). (b) Liver (Huh7, HepG2), muscle (C2C12, RD), brain (SVG-A), fibroblasts (Dermal fibs, BHKs), as well as Vero, HeLa and A549 cells were transfected with CHIKV-D-Luc-SGR RNA. Cell lysates were taken at indicated time points over a 48 h period and luciferase assayed (n = 3 experimental replicates, all luciferase signal normalised to a mock-transfected control). (c) Western blot of nsP3 in mammalian cell lines. Cells were transfected with wildtype (untagged) nsP3/SG-FLuc replicon, lysed at 24 hpt and Western blotted for CHIKV nsP3.