Figure 5.

CHIKV infection of mammalian cell lines. (a) Virus titres. Huh7, C2C12, SVG-A, and dermal fibroblast (D Fibs) cells were infected at MOIs of either 1 or 5. At 24 hpi, the media was collected and virus titred by plaque assay (n = 3, experimental replicates). (b) Corresponding RNA quantification of the infected cells. Intracellular RNA was extracted using TRIzol and quantified by qRT-PCR using primers specific for nsP3 (n = 3). Values in (a) and (b) were derived from the same number of cells and are thus directly comparable. (c) Western blots for nsP3 in infected cells. Cells were infected with wildtype CHIKV at an MOI = 1. Cells were lysed at 24 hpi and western blot conducted for nsP3.