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. 2017 Nov 7;7:14779. doi: 10.1038/s41598-017-14965-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Constitutive NIK expression expands ex-Foxp3⁺ T cells in vivo. CD4 T cells from NIKtg/Foxp3^Cre/R26^YFP and WT/Foxp3^Cre/R26^YFP littermates were assessed for percent ex-Foxp3⁺ T cells (defined as percent of total CD4⁺YFP⁺ cells that are Foxp3⁻). (a) Representative FACS plots from blood showing the gating scheme. (b) Percent ex-Foxp3⁺ T cells in blood over time. Each symbol and line represents an individual mouse. (c,d) Percent and number of ex-Foxp3⁺ T cells in indicated organs at euthanasia. mLN, mesenteric lymph nodes; pLN, peripheral lymph nodes. All data are from one representative experiment of 3. Bar graphs depict means +/− SD (n = 4 mice per group). *p < 0.05.