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. 2017 Nov 7;7:14751. doi: 10.1038/s41598-017-15281-0

Figure 3.

Figure 3

GeNorm expression stability analysis of the nine candidate reference genes, for which specific primers could be constructed. (a) Optimal number of reference genes for hPDL RT-qPCR data normalization in orthodontic studies (compressive orthodontic force vs. untreated control, n = 12), studies on periodontitis (Agac toxins vs. untreated control, n = 12) and pooled/overall (n = 18). (b) Average expression stability values of overall (pooled) specimens derived by stepwise exclusion of the least stable reference gene across all specimens and experimental conditions (n = 18). A smaller M value indicates a more stable gene expression. Gene names see Table 1.