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. 2017 Nov 7;7:14751. doi: 10.1038/s41598-017-15281-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup for the hPDL fibroblast experiments. (a) 6-well cell culture plate with untreated controls and simulated periodontitis (left side) as well as simulated orthodontic compressive force (right side). (b) Experimental conditions tested (physiological cell culture conditions): untreated physiological controls (adherently growing hPDL cells at 70% confluence in full medium), simulated orthodontic compressive force of 2 g/cm² applied by a 17.1 g glass disc, simulated periodontitis by adding bacterial lysate (toxins) of Aggregatibacter actinomycetemcomitans to the medium.