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. 2017 Nov 7;7:14769. doi: 10.1038/s41598-017-15039-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IgG Isotype specificity, surface expression of NS1 proteins, ADCC, complement fixation, and T cell responses of MVA-ZIKV-NS1 antisera. (a) The magnitude of Ig isotype specificity of immune sera from MVA-ZIKV-NS1 immunized mice was determined by ELISA, using rabbit anti-mouse immunoglobulin isotype-specific antibodies recognizing IgG1, IgG2a, IgG2b, IgG3, IgM, or IgA antibodies. (b) NS1-expression of inoculated Vero cells assayed by flow cytometric analysis. (c) ADCC activity observed following incubation of ZIKV-infected Vero cells (target cells) with serial dilutions of immune sera generated from MVA-ZIKV-NS1, Adeno-prME VLPs²⁴, or naïve sera in the presence of effector cells (Jurkat cell line). (d) Complement-mediated lysis of ZIKV-infected Vero cells. C′, native complement, ΔC′, heat inactivated complement. Naïve sera, sera from sham immunized mice. (e,f) T cell responses of immunized mice. (f) ICS analysis of CD8+ T cells demonstrated IL2 and IFNγ production in the CD8+ T cell subset 10 days post-vaccination. (e) No appreciable cytokine production was observed in CD4+ T cells by this time.