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. 2017 Nov 7;7:14625. doi: 10.1038/s41598-017-15212-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CL-11 and MASP-2 interaction on hypoxia-stressed iPS-RPE cells. (a) Representative Western blot showing MASP-2 protein expression in iPS-RPE cells under normal and hypoxic conditions. HSP90 was used as a loading control. Full-length blots are presented in Supplemental Fig. 6. (b) Relative density analysis of MASP-2 in iPS-RPE cells under different treatments. N = 3 independent experiments. (c) Representative immunofluorescence images showing MASP-2 staining on hypoxia-stressed iPS- RPE cells compared to non-stressed cells. MASP-2 (red) and phalloidin (aqua) are shown on the left panels and MASP-2 (red) alone is shown on the right panels. Scale bars, 25 μm. (d) Representative immunofluorescence images showing co-expression of CL-11 and MASP-2 (orange) on the surface of hypoxia-stressed iPS- RPE cells MASP-2 (red), CL-11 (green) nuclei (blue) and phalloidin (grey) are shown. Scale bars, 25 μm. (e) CL-11 and MASP-2 co-localization was quantified by calculating Mander’s co-localization coefficients using JACoP plug-in and ImageJ software.