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. 2017 Nov 6;149(11):1029–1039. doi: 10.1085/jgp.201711788

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© 2017 Hariharan and Guan

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Figure 4. — Determination of the absolute dissociation constants for Na⁺ (K_D(Na+)) and H⁺ (K_D(H+)). The apparent Na⁺-binding dissociation constants (K_d(Na+)) in the absence or presence of melibiose in the pH range from 5.55 to 8.45 were determined by ITC at 25°C. 1–20 mM NaCl was placed in the syringe. All apparent K_d(Na+) data were plotted against the H⁺ concentration without averaging and fitted by a linear function; the absolute K_D(Na+) value corresponds to the y-axis intercept, and the absolute K_D(H+) value corresponds to the x-axis intercept (see Materials and methods). pK_a = −log K_D(H+).