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. 2017 Nov 6;149(11):1029–1039. doi: 10.1085/jgp.201711788

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© 2017 Hariharan and Guan

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Figure 5. — Buffer effects on Na⁺ binding enthalpy (ΔH_ITC(Na+)). ITC was used to determine Na⁺ binding ΔH_ITC(Na+) to MelB_St in a total of five buffer systems in the absence of melibiose at pH 6.25, 7.45, and 8.2 at 25°C. 5–15 mM NaCl was placed in the syringe. (a) ΔG versus standard buffer −ΔH_(H+) plot. (b) ΔH_ITC(Na+) determined by ITC versus buffer standard −ΔH_(H+) plot, with linear fits to the data.