Table 1.
Correlation between pfhrp2 PCR result and Plasmodium falciparum parasitaemia.
| 18SrDNA + ve N = 131 | 18SrDNA + ve > 5 parasite/μl N = 91 | 18SrDNA/msp1/msp2 PCR + ve > 5 parasite/ul N = 15 | Mean parasite per µL (range) by qPCR1 | Mean parasite/µL by microscopy1 | P-value2 | |
|---|---|---|---|---|---|---|
| pfhrp2 + RDT − | 1 (0.8%) | 1 (1.1%) | 1 | 167 | 0 | 0.012a 0.482b |
| pfhrp2 + RDT + | 74 (56.4%) | 72 (79.1%) | NA | 689 (8.2–3923) | 1317 (0–7840) | |
| pfhrp2− RDT+ | 34(26%) | 10 (11%) | 8 | 1088 (8.5–6097) | 1540 (240–3560) | |
| pfhrp2− RDT− | 22 (16.8%) | 8(8.8%) | 6 | 10 (1.2–28.1) | 0 |
1For samples that were positive by both 18SrRNA and qPCR, with qPCR parasitaemia ≥5 parasites per µL.2 Kruskal-Wallis rank test for2a pfhrp2+ and pfhrp2− of all samples, and2b for samples with microscopically detected parasitaemia (i.e. excluding samples with submicroscopic parasitaemia). S18rDNA, 18 ribosomal RNA subunit gene; pfhrp2 and pfhrp3, Plasmodium falciparum histidine-rich protein 2 and 3 respectively; RDT, rapid diagnostic test.