Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 7;7:14744. doi: 10.1038/s41598-017-15241-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Detection of pms3 and tms5 genes in PS006 and PS012. (a) PCR product bands of pms3 in PS006 (1), PS012 (2), HD9802S (3) and PA64S (4). M: marker 2000. Fragment 1 is the first half of the pms3 gene (808-bp.) Fragment 2 is the second half of the pms3 gene (638-bp). The two fragments are spliced into a full pms3 gene (1236-bp). (b) PCR product bands of a 1060-bp fragment of the tms5 gene in PS006 (1), PS012 (2), HD9802S (3) and PA64S (4). This sequence was designed to contain the target site. M: marker 2000. (c) Sequence comparison of the pms3 gene in PS006, PS012, HD9802S, PA64S, NK58S and NK58. The mutation sites are marked in red. The mutated nucleotide positions (arrows) are shown at the top. (d) Sequence comparison of tms5 in PS006, PS012, HD9802S, PA64S, AnS-1 and AnN. The mutated nucleotide at positions 70 and 71 (arrow) are shown in red and bold.