Skip to main content
. 2017 Oct 4;17(10):2271. doi: 10.3390/s17102271

Figure 2.

Figure 2

Fabrication and assembly of the spheroid culture chamber array (SCCA): (a) The 3D printed molds of the thick and channel layers were screwed onto polymethylmetacrylate (PMMA) plates, polydimethylsiloxane (PDMS) at 10:1 was poured inside the molds, cured at 80 °C for 1 h and removed from the molds; (b) For the membrane layer, PDMS at 20:1 was poured over a silanized glass wafer before being rotated at 300 rpm for 1 min and cured at room temperature for 72 h; (c) The thick layer and the membrane layer (still mounted on its substrate) were plasma bonded together; (d) Holes corresponding to the inlets/outlets were punched into the membrane layer using the thick layer as a guide; (e) The other side of the membrane layer and the channel layer were plasma bonded together.