Figure 1.

The experimental design of this study. The initiator strain was the RJK56 rad27Δ haploid (Kokoska et al. 1998). Two subculture zero plates were used and streaked for single colonies on YPD and grown for ∼7 d. One colony from each of these plates was designated subculture one, and, after growing and streaking for singles, one colony from each plate was picked to be grown as subculture two. We repeated this process until subculture five. After subculture five was plated for single colonies, five independent colonies were picked, and the process was repeated until subculture 25, with each subculture grown for ∼3 d. Using a HiSeq2000 machine, we sequenced two lines, each with seven colonies, for a total of 14 strains. Each line contained one sample from subculture zero, one sample from subculture five, and five samples from subculture 25.