Figure 1.

Filamentation scoring range for liquid and solid filamentation conditions. C. albicans wild-type and 124 mutant strains were tested for their ability to filament in liquid and solid filamentation conditions. For both assays, cells grown overnight in YPD were centrifuged and washed 2× with PBS prior to the experiment. For the liquid assays, 10 µl of washed cells were added to a prewarmed glass-bottom microscopy dish with inducing media. Cells were imaged after 3 hr of incubation at 37° with shaking. For solid assays, 1 µl of washed cells was plated onto solid media and grown at 37° for 4–5 d before imaging. The images shown are representative images from the analysis at the respective scores for inducing conditions. The scoring range for Spider solid media is shown in Figure S7.