Figure 3.

Gug is involved in memory retention using ethanol choice memory assay. Proportion of eggs laid on 6% ethanol oviposition cap following wasp exposure after three 24-hr time points is shown. Wild-type flies continue to oviposit on 6% ethanol after wasp exposure (A). Gug/+ heterozygotes (B) and flies expressing a Gug^RNAi hairpin in the MB show impaired memory retention (C, D). MB switch flies fed vehicle control show wild-type memory (E), while flies expressing a Gug^RNAi via RU486 feeding show impaired memory retention (F). Error bars represent 95% confidence intervals (n = 10 biological replicates) (* P < 0.05).