Table 2. Differentially expressed miRNAs in sea cucumber respiratory tree among three different experiments (DO 2 mg/L, DO 4 mg/L, and DO 8 mg/L).
| miR_name | DO2 (Mean ± SD) | DO4 (Mean ± SD) | DO8 (Mean ± SD) | log2 [DO2 (Mean)/DO8 (Mean)] | log2 [DO2 (Mean)/DO4 (Mean)] | log2 [DO4 (Mean)/DO8 (Mean)] |
|---|---|---|---|---|---|---|
| Aja-miR-2008 | 3,046 ± 368 (b) | 833 ± 390 (a) | 949 ± 69 (a) | 1.6825 | 1.8710 | −0.1885 |
| Aja-miR-10-5p | 908,819 ± 99,048 (a) | 1,789,790 ± 217,900 (b) | 1,614,760 ± 388,785 (ab) | −0.8293 | −0.9777 | 0.1485 |
| Aja-miR-184 | 25 ± 5 (b) | 8 ± 5 (a) | 10 ± 5 (a) | 1.2841 | 1.6776 | −0.3935 |
| Aja-miR-71b | 8 ± 8 (a) | 34 ± 6 (b) | 18 ± 5 (a) | −1.1502 | −2.0310 | 0.8808 |
| Aja-miR-125-5p | 132,231 ± 32,089 (a) | 355,285 ± 71,455 (b) | 292,087 ± 14,330 (b) | −1.1433 | −1.4259 | 0.2826 |
| novel-miR-1 | 47 ± 11 (b) | 0.01 ± 0 (a) | 0.01 ± 0 (a) | 12.1984 | 12.1984 | 0.0000 |
| Aja-let-7a-5p | 8 ± 9 (a) | 35 ± 9 (b) | 28 ± 5 (b) | −1.7813 | −2.1054 | 0.3240 |
| Aja-miR-375-3p | 31,748 ± 5,645 (a) | 15,970 ± 6,033 (b) | 23,608 ± 8,389 (ab) | 0.4274 | 0.9914 | −0.5639 |
| Aja-miR-2013-3p | 606 ± 72 (a) | 1,427 ± 292 (b) | 1,331 ± 68 (b) | −1.1339 | −1.2352 | 0.1012 |
| novel-miR-2 | 33 ± 11 (b) | 0.01 ± 0 (a) | 0.01 ± 0 (a) | 11.6883 | 11.6883 | 0.0000 |
| Aja-miR-2835 | 54 ± 20 (b) | 0.01 ± 0 (a) | 0.01 ± 0 (a) | 12.3987 | 12.3987 | 0.0000 |
| Aja-miR-1 | 66 ± 24 (b) | 9 ± 1 (ab) | 7 ± 5 (a) | 3.2340 | 2.9164 | 0.3175 |
| Aja-miR-71-5p | 58,756 ± 13,628 (a) | 146,865 ± 44,428 (ab) | 115,157 ± 12,697 (b) | −0.9708 | −1.3217 | 0.3509 |
| Aja-miR-200-3p | 35,729 ± 3,207 (a) | 95,032 ± 34,864 (ab) | 102,503 ± 21,706 (b) | −1.5205 | −1.4113 | −0.1092 |
| Aja-miR-2011-3p | 49,272 ± 4,264 (a) | 63,920 ± 17,541 (ab) | 71,512 ± 1,331 (b) | −0.5374 | −0.3755 | −0.1619 |
| Aja-miR-2478a | 30 ± 23 (a) | 124 ± 117 (ab) | 151 ± 37 (b) | −2.3305 | −2.0527 | −0.2778 |
| Aja-miR-31-5p | 9,141 ± 418 (a) | 9,179 ± 4,431 (ab) | 11,421 ± 819 (b) | −0.3212 | −0.0059 | −0.3153 |
| Aja-miR-7977 | 0.01 ± 0 (a) | 4 ± 5 (ab) | 6 ± 2 (b) | −9.2288 | −8.6439 | −0.6406 |
| Aja-miR-71a | 1,792 ± 345 (a) | 3,346 ± 3,226 (ab) | 2,688 ± 346 (b) | −0.5848 | −0.9008 | 0.3160 |
| Aja-miR-29b-3p | 3,179 ± 241 (b) | 2,828 ± 799 (ab) | 2,400 ± 345 (a) | 0.4055 | 0.1690 | 0.2365 |
| Aja-miR-2478b | 0.01 ± 0 (a) | 1 ± 2 (ab) | 2 ± 1 (b) | −7.6439 | −6.6439 | −0.8295 |
| Aja-miR-2008-5p | 88 ± 34 (a) | 171 ± 114 (ab) | 190 ± 48 (b) | −1.1100 | −0.9636 | −0.1464 |
| Aja-miR-1a-3p | 101,709 ± 26,157 (ab) | 77,382 ± 10,825 (b) | 55,209 ± 7,288 (a) | 0.8815 | 0.3944 | 0.4871 |
| novel-miR-3 | 27 ± 24 (ab) | 5 ± 1 (a) | 0.01 ± 0 (a) | 11.3987 | 2.5481 | 8.9658 |
| Aja-miR-153-3p | 178 ± 106 (ab) | 39 ± 7 (a) | 57 ± 3 (b) | 1.6360 | 2.1911 | −0.5551 |
| Aja-miR-153 | 23 ± 17 (ab) | 0.01 ± 0 (a) | 4 ± 1 (b) | 2.6895 | 3.3617 | −8.6439 |
Values indicate the means ± SD (n = 3). Means not sharing a given letter (a and b) differ significantly (P < 0.05).