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Absorption and fluorescence properties of the fluorophore-modified ssDNAs P1-FAM, P2-TAMRA, and P3-Cy5. (A) Sequence of the three ssDNAs and the modified fluorophores. (B) Normalized UV-VIS absorption and emission spectra of P1-FAM, P2-TAMRA, and P3-Cy5 in PBS (pH = 7.4). The λex/λem combinations to acquire fluorescence signatures are shown in the upper panel.
