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. Author manuscript; available in PMC: 2018 Nov 7.
Published in final edited form as: Structure. 2017 Oct 19;25(11):1719–1731.e4. doi: 10.1016/j.str.2017.09.009

Figure 1. Binding of N12-i3 to EGFP-CEM-NKr-CCR5-SNAP cells infected with BaL infectious molecular clone (IMC).

Figure 1

EGFP-CEM-NKr-CCR5-SNAP were infected with IMC BaL virus and evaluated 5-days post-infection for (A) intracellular p24, cell surface levels of CD4 and (B) the binding of: N12-i3, Cluster A mAbs A32, N5-i5 and C11, and Synagis. Panel A shows the gating strategy for EGFP-CEM-NKr-CCR5-SNAP Bal-infected (Blue gates) and Mock (Red gate) cells, previously defined as GFP+ and L/D dye- cells. For Bal-infected cells two gates were delineated: p24+/CD4- and p24+low/CD4+; one gate was delineated for Mock p24-/CD4+. (C) ADCC using the RFADCC method. The curves shown are normalized for plateau cytotoxicity values using C11.