KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER (RRID) |
|---|---|---|
| Antibodies | ||
| Mouse anti-beta III tubulin (801201) | Biolegend | RRID:AB_2313773 |
| Mouse Anti-Glial Fibrillary Acid Protein (G3893) | Sigma | RRID:AB_2313773 |
| Rabbit Anti-Glial Fibrillary Acid Protein | EnCor Biotechnology | RRID:AB_2572310 |
| Goat anti-FATP1 | Santa Cruz Biotechnology | RRID:AB_2239414 |
| Goat anti-FATP4 (SC-5835) | Santa Cruz Biotechnology | RRID:AB_2190629 |
| Mouse Anti-6x-His Epitope Tag (HIS.H8) | ThermoFisher | RRID:AB_2313773 |
| Rabbit Anti-Human APOE | Abcam | RRID:AB_867703 |
| Mouse Anti-Actin (C4) | EMD Millipore | RRID:AB_2223041 |
| Rabbit P75-NTR | Biolegend | RRID:AB_2565441 |
| Rabbit Anti-active Caspase 3(Asp175) | Cell Signaling | RRID:AB_443014 |
| Bacterial and Virus Strains | ||
| Lentiguide-Puro-BFP | This manuscript | Backbone obtained from addgene: Plasmid # 52963 Plasmid #34632 |
| Stbl3 competent cells | Thermo Fisher | Cat # C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Nile Red | Sigma | Cat # 72485 |
| BODIPY (493/503) | Thermo Fisher | Cat # D3922 |
| SR 13800 (MCTi2) | Tocris | Cat # 5431 |
| AR-C155858 (MCTi) | Tocris | Cat # 4960 |
| Rotenone | Sigma | Cat # R8875 |
| Sodium L-Lactate | Sigma | Cat # 71718 |
| Dichloroacetic acid (PESTANAL® analytical standard) | Aldrich | Cat # 36545 |
| U-13C-d-Glucose | Cambridge Isotopes | Cat # CLM-1396-PK |
| TrypLE | ThermoFisher | Cat # 12605010 |
| SytoxRed | ThermoFisher | Cat # S34859 |
| Critical Commercial Assays | ||
| In Situ Cell Death Detection Kit, Fluorescein | Roche | Cat # 11684795910 |
| SuperSignal™ West Pico Chemiluminescent Substrate | ThermoFisher | Cat # 34080 |
| Experimental Models: Organisms/Strains | ||
| y w*; P{GAL4-elav.L}2 | Drosophila melanogaster | BDSC_8765 |
| y1 w*; P{w[+m*]=GAL4}54C | Drosophila melanogaster | BDSC_27328 |
| y1 w*; P{nSyb-GAL4.S}3 | Drosophila melanogaster | BDSC_51635 |
| y1 w*; P{w+mC=UAS-mCD8::GFP.L}LL5 | Drosophila melanogaster | BDSC_5137 |
| w*; P{w+mC = UAS-bsk.B}2 | Drosophila melanogaster | BDSC_9310 |
| y w; P{UAS-SREBP.K}2/CyO | Drosophila melanogaster | BDSC_38396 |
| P{NinaE-GD6220} (Rh-ND42 RNAi) | Drosophila melanogaster | (Liu et al., 2015) |
| P{NinaE-GD11094} (Rh-Marf RNAi) | Drosophila melanogaster | (Liu et al., 2015) |
| P{NinaE-KK108492} Rh-Aats-Met RNAi) | Drosophila melanogaster | (Liu et al., 2015) |
| P{KK104306}VIE-260B (Sln RNAi) (Volkenhoff et al., 2015) | Drosophila melanogaster | FlyBase_FBst0481152 |
| w1118; P{GD1940}v4607 (Sln RNAi) | Drosophila melanogaster | FlyBase_FBst0466501 |
| w1118; P{GD3448}v51157 (out RNAi) (Volkenhoff et al., 2015) | Drosophila melanogaster | FlyBase_FBst0469314 |
| P{KK104187}VIE-260B (out RNAi) (Volkenhoff et al., 2015) | Drosophila melanogaster | FlyBase_FBst0480175 |
| w[1118]; P{GD12666}v26802/CyO (shakB RNAi) (Pezier et al., 2016) | Drosophila melanogaster | FlyBase_FBst0456596 |
| y1 sc* v1; P{y+t7.7 v+t1.8=TRiP.HMC04895}attP2 (shakB RNAi)(Pezier et al., 2016) | Drosophila melanogaster | BDSC_57706 |
| y1 sc* v1; P{TRiP.HMC03111}attP2 (Fatp RNAi) | Drosophila melanogaster | BDSC_50709 |
| y1 sc* v1; P{TRiP.HMC03960}attP40 (Fatp RNAi) | Drosophila melanogaster | BDSC_55273 |
| y1 sc* v1; P{TRiP.HMC04206}attP2 (Fatp RNAi) | Drosophila melanogaster | BDSC_55919 |
| P{KK104809}VIE-260B (Fatp RNAi) (Dourlen et al., 2012) | Drosophila melanogaster | FlyBase_FBst0471998 |
| y1 v1; P{TRiP.HMS00039}attP2 (Ldh RNAi) | Drosophila melanogaster | BDSC_33640 |
| P{KK107553}VIE-260B (Nlaz RNAi) | Drosophila melanogaster | FlyBase_FBst0473194 |
| w1118; P{GD12709}v35558 (Nlaz RNAi) | Drosophila melanogaster | FlyBase_FBst0461214 |
| P{KK107553}VIE-260B (Nlaz RNAi) | Drosophila melanogaster | FlyBase_FBst0473194 |
| w1118; P{GD4806}v15387/TM3 (Glaz RNAi) | Drosophila melanogaster | FlyBase_FBst0451831 |
| w1118; P{GD4806}v15389/TM3 (Glaz RNAi) | Drosophila melanogaster | FlyBase_FBst0451832 |
| P{KK106377}VIE-260B (Glaz RNAi) | Drosophila melanogaster | FlyBase_FBst0479254 |
| P{KK101856}VIE-260B (Pdha RNAi) | Drosophila melanogaster | FlyBase_FBst0479031 |
| w1118; P{GD12103}v40410 (Pdha RNAi) | Drosophila melanogaster | FlyBase_FBst0463545 |
| w1118; P{GD15718}v43306 (Bsg RNAi) | Drosophila melanogaster | FlyBase_FBst0465019 |
| w1118; P{GD15718}v43307(Bsg RNAi) | Drosophila melanogaster | FlyBase_FBst0465020 |
| w1118; P{GD989}v2789 (Bsg RNAi) | Drosophila melanogaster | FlyBase_FBst0457179 |
| P{w+mC=GMR-hid}SS1, y1 w* P{ry+t7.2=neoFRT}19A; P{ w+m*=GAL4-ey.H}SS5, P{w+mC=UAS-FLP1.D}JD2 | Drosophila melanogaster | BDSC_5248 |
| y1 w* sicilyE P{ry+t7.2=neoFRT}19A/FM7c, P{w+mC=GAL4-Kr.C}DC1, P{w+mC=UAS-GFP.S65T}DC5, sn+ | Drosophila melanogaster | BDSC_52394 |
| y1 w* MarfB P{ry+t7.2=neoFRT}19A/FM7c, P{w+mC=GAL4-Kr.C}DC1, P{w+mC=UAS-GFP.S65T}DC5, sn+ | Drosophila melanogaster | BDSC_67154 |
| P{lacW}BsgSH1217P{neoFRT}40A/CyO | Drosophila melanogaster | (Besse et al., 2007) |
| y1 w*; Mi{Trojan-GAL4.no-pA.0}GLazMI02243 | Drosophila melanogaster | This paper |
| w1118; NlazNW5 | Drosophila melanogaster | (Hull-Thompson et al., 2009) |
| w*; SlnD1/CyO | Drosophila melanogaster | (Jang et al., 2008) |
| y1 w* l(1)G0334A P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn+ | Drosophila melanogaster | BDSC_52370 |
| y1 w* kdnA P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn+ | Drosophila melanogaster | BDSC_52364 |
| yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Fatpk10307 P{neoFRT}40A/CyO y+ | Drosophila melanogaster | BDSC_10988 |
| y1 w*; PBac{UAS-APOE2.C112, C158}VK00033 | Drosophila melanogaster | This paper |
| y1 w*; PBac{UAS-APOE3.C112, R158}VK00037 | Drosophila melanogaster | This paper |
| y1 w*; PBac{UAS-APOE4.R112, R158}VK00037 | Drosophila melanogaster | This paper |
| B6.129P2-Apoetm1Unc/J | Mus musculus | IMSR_JAX:002052 |
| Gt(ROSA)26Sortm1.1(CAG-cas9*,-EGFP)Fezh/J | Mus musculus | IMSR_JAX:024858 |
| Tg(Aldh1l1-EGFP,-DTA)D8Rth | Mus musculus | IMSR_JAX:026033 |
| C57BL/6J | Mus musculus | IMSR_JAX:000664 |
| FATP1 | CACCGGTCGTTTGCCACCGTTAGAGTGG | n/a |
| FATP1 | CACCGTCGTTTGCCACCGTTAGAGTGGG | n/a |
| FATP4 | CACCGAGGTAGCGTTGTGCCTCTACCGG | n/a |
| FATP4 | CACCGTATGTCCCCACGACGAGGCTTGG | n/a |
| Nontarget | CACCGGCGAGGTATTCGGCTCCGCGTGG | n/a |
| Recombinant DNA | ||
| APOE3 | Harvard Human cDNA collection | HsCD00323815 |
| Software and Algorithms | ||
| ImageJ | NIH | n/a |
| Illustrator CS6 | Adobe | n/a |
| Photoshop CS5 | Adobe | n/a |
| GraphPad Prism 7 | GraphPad software | n/a |
| Microsoft Excel 2016 | Microsoft Corporation | n/a |