Fig. 3.

T cell infiltration in PDAC is regulated by CTLA-4/CD80. Shown is expression of: a CD80, and b CD86 on dendritic cells (CD11c⁺ F4/80^neg), granulocytes (CD11b⁺ Gr-1⁺), and macrophages (F4/80⁺ CD11c^neg Gr-1^neg) from peritumoral lymph nodes in KPC mice (n = 7). c Comparison of CD80 and CD86 expression frequency on CD11c⁺F4/80^neg cells in the lymph node. d Shown are representative immunohistochemistry images of peritumoral lymph nodes from KPC mice at 14 ± 2 days after treatment with control or anti-CD25. Tissues are co-stained for CD11c (brown) and Foxp3 (black). e Quantification of Foxp3⁺ Treg per high power field (hpf) in peritumoral lymph nodes of control (n = 3) and anti-CD25 (n = 5) antibody-treated KPC mice. f Quantification per hpf and g representative immunohistochemistry images of CD3, CD4, CD8 and Foxp3 cells detected in PDAC tumors from KPC mice (n = 3–5 mice/group) treated with anti-CD80 antibody versus control. Statistical significance was determined by one-way ANOVA and Tukey’s post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001