Figure 1. Characterization of 3D encapsulated human monocytic THP-1 cells behavior in IL-4 incorporated PEGDA and GelMA hydrogels for 6 days.
(A) THP-1 cells morphology in IL-4 cytokine incorporated PEGDA and GelMA hydrogels. (B) Quantitation of cellsdiameter in IL-4 cytokine incorporated PEGDA or GelMA hydrogel. (C) Distribution of cell diameter in IL-4 cytokine incorporated PEGDA and GelMA hydrogels. (D) Micrographs of cellsin hydrogel constructs stained for M1 surface marker CD86 (red) and M2 surface marker CD206 (green). Scale bar represents 100 μm. (E) Quantitative analysis of micrographs. (F) Real-time PCR of M1 related IRF5 and IL6 and (G) M2 related STAT6 and IL10. Scale bar represents 100 μm. The data are shown as the mean ± SEM (*, p < 0.05).