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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Eur J Cell Biol. 2017 Oct 13;96(8):758–766. doi: 10.1016/j.ejcb.2017.10.001

Figure 3.

Figure 3

miR-200b alters the expression of KLF2. (A) HUVECs were transfected with miR-200b mimic or antagomiR, and the mRNA levels were monitored in normoxic conditions and after 4 h exposure to hypoxia. KLF2 mRNA levels from 3 independent experiments (n=12) are plotted normalized to 18S rRNA or TBP mRNA levels and expressed as a fold change over the normoxic control. Significant changes (p<0.05) are marked with an asterisk. (B) The corresponding changes of Klf2 protein levels were detected with SDS-PAGE and Western Blot and normalized to the β-Actin and 2 individual samples (4 µg of total protein per lane) were tested for each treatment and the experiments were repeated twice.