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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Eur J Cell Biol. 2017 Oct 13;96(8):758–766. doi: 10.1016/j.ejcb.2017.10.001

Figure 4.

Figure 4

miR-200b binds to predicted target sequence in the KLF2 3’UTR (A).The RNAhybrid predicted miR-200b target site at 3' UTR of KLF2 mRNA is presented at the top right. HUVECs were transfected with KLF2 target sequence-specific target protector and/or the miR-200b analog (Mimic 200b) and exposed to hypoxia for 4 h. The KLF2 mRNA levels were monitored in qRT-PCR experiments. The KLF2 levels results from 2 independent experiments (n=8) are plotted normalized to 18S rRNA levels and expressed as a fold change over the transfection control. Significant changes (p<0.05) are marked with an asterisk. (B). The corresponding changes of Klf2 protein levels were detected with SDSPAGE and Western Blot and normalized to the β-actin levels 2 µg of total protein per lane was loaded for each sample and the experiments were repeated twice.