Table 1. Genes related to the hypoxia-induced angiogenesis that were altered in HUVECs following miR-200b reduction/induction.
The genes were preselected based on RNASeq experiments and subsequent Gene Ontology analysis. The presence of potential miR-200b target sequences was predicted in bioinformatics approach (Betel et al., 2008; Kruger and Rehmsmeier, 2006; Lewis et al., 2005). The changes in genes expression after miR-200b overexpression and inhibition were accessed by qPCR in normoxia and under hypoxia (Supportive Figure 2). The previously proposed miR-200b targets that are angiogenesis related were included as well and denoted with an asterisk. Opposite mRNA expression patterns after overexpression or inhibition of miR-200b are highlighted in grey.
| Gene | miR- 200b target site |
miR-200b effect in normoxia | miR-200b effect under hypoxia |
Role in angiogenesis |
Ref. |
|---|---|---|---|---|---|
| KLF2 | yes | Downregulated with mimic and upregulated with antagomiR | Downregulated with mimic and upregulated with antagomiR | antiangiogenic | (Bhattacharya et al., 2005; Kawanami et al., 2009) |
| *KDR | yes | Downregulated with mimic and upregulated with antagomiR | Downregulated with mimic and not changed with antagomiR | proangiogenic | (Soldi et al., 1999) |
| *ETS1 | yes | Downregulated with mimic and antagomiR | Downregulated with mimic and antagomiR | proangiogenic | (Hashiya et al., 2004) |
| *VEGFA | yes | Downregulated with mimic and antagomiR | Downregulated with mimic and antagomiR | proangiogenic | (Ferrara et al., 2003) |
| NOS3 | yes | Downregulated with mimic only | Upregulated with antagomiR | proangiogenic | (Amano et al., 2003) |
| THSD7A | yes | Downregulated with mimic | No significant effects | antiangiogenic | (Wang et al., 2010) |
| ITGB3 | yes | Downregulated with mimic | Downregulated with mimic | proangiogenic | (Brooks et al., 1994) |
| MYOF | yes | Downregulated with mimic | No significant effects | proangiogenic | (Yu et al., 2011) |
| NRP2 | yes | Downregulated with mimic | Downregulated with mimic | proangiogenic | (Takashima et al., 2002) |
| FYN | yes | Downregulated with mimic and antagomiR | Downregulated with mimic | proangiogenic | (Sen and Johnson, 2011) |
| FZD4 | yes | Downregulated with mimic | Downregulated with mimic | proangiogenic | (Chen et al., 2011a) |
| GRB10 | yes | Downregulated with mimic | Downregulated with mimic | proangiogenic | (Soriano et al., 2004) |
| EP300 | yes | Downregulated with mimic and antagomiR | Downregulated with mimic and antagomiR | proangiogenic | (Zhang et al., 2013a) |
| *FLT1 | yes | No effect | Downregulated with mimic and antagomiR | proangiogenic | (Birnbaum, 1995) |
| RGS5 | yes | Downregulated with mimic | Downregulated with mimic | proangiogenic | (Mitchell et al., 2008) |
| HMOX1 | yes | Downregulated with mimic and antagomiR | Downregulated with mimic and antagomiR | proangiogenic | (Malaguarnera et al., 2002) |