REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Myc-tag-488 monoclonal antibody, Clone 4A6	EMD-Millipore	Cat# 05-724
Alexa-Fluor 488 labeled goat anti-mouse IgG mAb	Molecular Probes (ThermoFisher)	Cat# R37120
Bacterial and Virus Strains
DH5α	ThermoFisher	Cat# 18258012
DH10Bac	ThermoFisher	Cat# 10361012
Biological Samples
Human O erythrocytes	Vaccine Center at Emory University	N/A
Human B-cell line Tn4	(Mi et al., 2012)	N/A
Chemicals, Peptides, and Recombinant Proteins
RNeasy mini kit	Qiagen	Cat No./ID: 74104
SuperScript III Reverse Transcriptase	ThermoFisher
EZ-link Sulfo-NHS-LC-Biotin	ThermoFisher	Cat# 21335
QuickChange Lightning Multi Mutagenesis Kit	Agilent Technologies	Cat# 210515
UEA-1	Vector Laboratories	Cat# L-1060
H-trisaccharide Type II	Sigma	Cat# F7297
LNnT	Consortium for Functional Glycomics	N/A
2′-fucosyllactose	Consortium for Functional Glycomics	N/A
Deposited Data
Crystal Structure, RBC36 (APO)	This paper	PDB 5UFD
Crystal Structure, RBC36 + 2′-fucosyllactose	This paper	PDB 5UFF
Crystal Structure, O13 (APO)	This paper	PDB 5UEI
Crystal Structure, O13 + H-trisaccharide	This paper	PDB 5UF1
Crystal Structure, O13 + LNnT	This paper	PDB 5UF4
Crystal Structure, Tn4-22 (APO)	This paper	PDB 5UFB
Crystal Structure, Tn4-22 + H-trisaccharide	This paper	PDB 5UFC
Experimental Models: Cell Lines
Human cells: HEK-293F (Freestyle 293F)	ThemoFisher	Cat# R79007
Human cells: HEK-293S (GnTI−)	ATCC	RRID:CVCL_A785
Insect cells: Sf9 (Spodoptera frugiperda)	ThermoFisher	Cat# 11496015
Insect cells: Hi5 cells (Trichoplusia ni)	ThermoFisher	Cat# B85502
Experimental Models: Organisms/Strains
Petromyzon marinus	Lamprey Services	Larval lampreys
Oligonucleotides
VLRb LRRNT: GCATGTCCCTCGCAGTG	IDT	www.idtdna.com
VLRb LRRCT: CGTGGTCGTAGCAACGTAG	IDT	www.idtdna.com
O13-N81H, N82Q mutagenesis primer: CAGACCCTGTGGCTGCACCAGAACCAGCTGACCTCC	IDT	www.idtdna.com
Recombinant DNA
Plasmid: pCT-ESO-BDNF YSD vector	(Burns et al., 2014)	Gift from Eric Shusta
Plasmid: pCDH	Systems Biosciences	Cat# CD500A-1
Plasmid: pFastBac1	ThermoFisher	Cat# 10360014
Plasmid: phCMV3	Amsbio	P003300
Software and Algorithms
HKL2000	(Otwinowski and Minor, 1997)	hkl-xray.com/hkl-2000
XDS	(Kabsch, 2010)	xds.mpimf-heidelberg.mpg.de/
PHENIX	(Adams et al., 2010)	hwww.phenix-online.org/
Coot	(Emsley et al., 2010)	www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PDB validation server	PDB	validate.wwpdb.org
Other
CFG Glycan array	Consortium for Function Glycomics	Versions 5.0, 5.1
Stanford Synchrotron Radiation Lightsource (SSRL)	Stanford	Beamline 12-2
Advanced Photon Source (APS)	Argonne National Lab	23ID-B, 23ID-D