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. 2017 May 23;2:14. doi: 10.1038/s41536-017-0017-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Production of macrophages from human PSCs. Macrophage derived from hPSC express both CD11b and 25F9 (b) but not CD93 (a) and key M1 (c, d) and M2-associated genes (e, f) are upregulated upon stimulation. Assessed using a live imaging phagocytosis assay, naïve and M2-activated macrophages were more phagocytic than M1-activated macrophages and this was observed for both MDMs (g) and iPSC-DMs (h). [*p < 0.05, **p < 0.01, ***p < 0.001; n = 3, c–f One-way ANOVA, g, h Two-way ANOVA with Tukey’s multiple comparison test]