Fig. 3.

Mutation detection using GeneRead DNAseq targeted CRC panel sequencing. a Mutation detection in HCT116 cancer cells. Forty nanogram of DNA extracted from fresh HCT116 cells were subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were called using Ingenuity variant analysis software and compared to results reported from Cosmic* (catalog of somatic mutations in cancer) and ATCC websites. b Mutation detection in CRC patient biopsy tissues. Forty nanogram of DNA extracted from primary tumor and liver metastasis were subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding Mutation Allele Fraction (MAF) were analyzed and compared between primary tumor and liver metastasis. In both tables, the blue color highlights the detected mutation and the number inside each cell represents the MAF of the mutation