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. 2017 Nov 1;2:34. doi: 10.1038/s41525-017-0034-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Mutation detection in HCT116 cancer cells with different DNA input using GeneRead DNAseq targeted CRC Panel. Forty and 1 ng DNA extracted from fresh HCT116 cells were compared to 1.0, 0.5, and 0.2 ng of DNA extracted from HCT116 cells fixed with 4% PFA. In all conditions, DNA was subjected to multiplex PCR, library preparation and MiSeq sequencing. Mutations and corresponding allele frequencies were analyzed and compared