Fig. 2.

Myogenic and non-myogenic clonal c-kit-BMCs. a Sorted GFP-positive-c-kit-BMCs, plated at limiting dilution in semi-solid medium, generate single cell-derived clones (upper panels, phase contrast micrographs; lower panels, native GFP fluorescence). Scale bars: first panel = 50 µm; second panel = 100 µm; third and fourth panels = 200 µm. b Scatter plots of c-kit and GFP expression in clonal c-kit-BMCs. The numbers in the boxes correspond to the sampled cell clones. c Three weeks after myocardial infarction and injection of clonal GFP-positive-c-kit-BMCs, sites of viral integrations were detected in aliquots of the delivered cells and in isolated regenerated cardiomyocytes. The PCR products correspond to the sites of integration of the viral genome in the DNA of c-kit-BMCs and cardiomyocytes. Molecular mass: 100 bp incremental ladders