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. 2017 Sep 5;10(4):321–324. doi: 10.21053/ceo.2017.00129

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Copyright © 2017 by Korean Society of Otorhinolaryngology-Head and Neck Surgery

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Immunohistochemical labelling of calcitonin gene-related peptide (CGRP) using an avidin-biotin peroxidase complex method in the external auditory canal skin. (A) CGRP positive staining is prominently noted in the suprabasal portion of epithelium (×100). (B) Positive stained cells contain granular and prickle cell layers, in the epithelium (black arrows) (×200). (C, D) Reactions for CGRP were generally moderate in the secretory cells (black arrows), with their myoepithelial cells (white arrows) showing a stronger reaction. The CGRP staining in the sebaceous gland (sg) is somewhat weak, compared to other regions. ep, epithelium; dm, dermis; cg, ceruminous gland. (C, ×100; D, ×200).