Figure 4. Hepatic and intestinal expression of FXR and SHP in bile-diverted rats.
(a) Relative mRNA level and western blot of FXR in the liver. (b) Quantification of hepatic Shp mRNA level. (c–d) Relative mRNA levels of Fxr and Shp in the intestine of rats with bile diversion in a mesenteric vein and (e–f) in the mid-ileum. The site of bile reinsertion is indicated by a grey arrow for the mid-ileum bile diversion. Data were obtained 13 days after surgery in standard-fed rats in the post absorptive state and are expressed as mean + SEM; n=4–7 rats per group; *p<0.05, **p<0.01, ***p<0.001 vs sham-operated group (One-way ANOVA followed by Tukey’s post-hoc test for the studies in the liver and t test for the quantification in the intestine).