REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-mCherry 1/300	Clontech	Cat#632496; RRID: AB_10013483
Chicken polyclonal anti-GFP 1/1000	Aves lab	Cat#GFP-1020; RRID: AB_10000240
Mouse monoclonal (IgG2a) anti-PCNA	Santa Cruz	Cat# sc-56 AB-16.0387; RRID: AB_628110
Rabbit polyclonal (IgG2a) anti-PCNA 1/500	GeneTex	Cat#GTX124496 AB-16.0386; RRID: AB_11161916
Mouse monoclonal (IgG1) anti-Flag 1/500	Sigma	Cat#F1804 AB-16.0331; RRID: AB_262044
Mouse monoclonal (IgG2a) anti-GS 1/1000Clone GS-6	Millipore	Cat#MAB302 AB-16.0342
Human polyclonal anti-Hu 1/10000	Gift from B. Zalk	AB-16.0356
Anti-Parvalbumine 1/2000	Millipore	Cat#MAB1572 AB-16.0382
Chemicals, Peptides, and Recombinant Proteins
4-Hydroxytamoxifen	Sigma-Aldrich	T176; CAS: 68392-35-8
9-tert-ButylDoxycycline, hydrocloride salt	Echelon Biosciences	B-0801; CAS: 233585-94-9
DAPI	Sigma-Aldrich	D9542; CAS: 28718-90-3
Critical Commercial Assays
Taq DNA polymerase	EmeraldAmp Takara	Cat# 330A
Experimental Models: Organisms/Strains
Zebrafish: Tg(T2Kβactin:lox-stop-lox-hmgb1-mcherry)jh15	[38]	ZFIN ID: ZDB-TGCONSTRCT-110221-4
Zebrafish: Tg(her4.1:EGFP)y83	[25]	ZFIN ID: ZDB-FISH-150901-14830
Zebrafish: Tg(−3her4.3:ERT2-Cre-ERT2)vu298a	[33]	N/A
Zebrafish: Tg(-3.5ubi:loxP-GFP-loxP-mCherry)	[72]	ZFIN ID: ZDB-ALT-070612-3
Zebrafish: Tg(ubb:LOX2272-LOXP-RFP-LOX2272-CFP-LOXP-YFP)a131, ubi:Zebrabow-M	[73]	ZFIN ID: ZDB-ALT-110825-11
Zebrafish: Tg(her4:rtTA, cmlc2:GFP)	[71]	ZFIN ID: ZDB-PUB-101209-26
Zebrafish: Tg(GFP:biTRE:H2amCherry,crist:Venus)	[36]	ZFIN ID: ZDB-ALT-130816-2
Oligonucleotides
rtTA probe fw: ATGTCTAGACTGGACAAGAGCAAAGTC, rv:GGATCCATTAACCCTCACTAAAGGGACTAACTGTCGACCTTGTC.	N/A	N/A
her4AttB4-fw: GGGGACAACTTTGTATAGAAAAGTTGTTTTGCATTATTTCCCTAATTTTAAATGTC (−999-1028bp from AY691485.1) her4AttB1-Rv: GGGGACTGCTTTTTTGTACAAACTTGGTCAGGATCAGATCTGAGCTG (−3389-3409bp from AY691485.1)	N/A	N/A
Recombinant DNA
her4:rtTA,GFP:cmlc2	This paper	N/A
GFP:biTRE:H2amCherry,crist:Venus	This paper	N/A
tetOn Toolkit	[31]	https://www.bio.umass.edu/biology/jensen/node/5
Tol2kit	[72]	http://tol2kit.genetics.utah.edu/index.php/Main_Page
Software and Algorithms
Imaris (Spot Segmentation)	Bitplane	http://www.bitplane.com/imaris/imaris
Photoshop CS6	N/A	N/A
ZEN Black software	Carl Zeiss, Germany	https://www.zeiss.fr/microscopie/produits/microscope-software/zen.html
MATLAB 2014b, 2015b (Data analysis)	MathWorks	http://www.mathworks.com