Constitutive Expression of ZmG2 or ZmGLK1 Leads to Enhanced Greening of Regenerating Callus and T1 Seedlings
(A–G) Representative regenerating callus of T0 cultivar IR64 lines: wild-type (WT) (A), ZmPEPCpro:ZmG2 (B), ZjPCKpro:ZmG2 (C), ZmUBIpro:ZmG2 (D), ZmPEPCpro:ZmGLK1 (E), ZjPCKpro:ZmGLK1 (F), and ZmUBIpro:ZmGLK1 (G).
(H–J) Representative regenerating callus of T0 cultivar Kitaake lines on cytokinin-containing media: WT (H), ZmUBIpro:ZmG2 (I), and ZmUBIpro:ZmGLK1 (J).
(K–P) Representative T1 seedling shoots (K, M, and O) and roots (L, N, and P) of IR64 lines: WT (K and L), ZmUBIpro:ZmG2 (M and N), and ZmUBIpro:ZmGLK1 (O and P). Yellow arrows indicate hypocotyl, white arrows indicate primary root.
Scale bars, 0.5 cm (A–G), 0.8 cm (H–J, L, N, and P), and 0.2 cm (K, M, and O). See also Figure S1 and Table S1.