Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 8;7:15103. doi: 10.1038/s41598-017-15128-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The US9-driven TEV protease expression correlates with substrate processing. (a) Electrophoretic analysis of proteins extracted from 293T cells co-transfected with a constant amount of the substrate g9tcsmC and increasing amounts (ranging from 0 to 150 ng) of the protease 9t. The presence of uncleaved and cleaved substrate was revealed with the anti-mCherry antibody. In this range of expression, the increasing presence of the US9-driven protease correlates with a constant decrease of the full length substrate and the corresponding increase of the processed fragment. The densitometric analysis of the same blot was used to generate the histogram shown in (b). The height of the columns corresponds to the % of cleavage in each individual sample, calculated as described in Results and in the legend to Figure S2b. The full-length blot is presented in the Supplementary material (S7).