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. 2017 Nov 8;7:15045. doi: 10.1038/s41598-017-13755-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic description of the transduction protocol. (a–d) Illustration of the four main steps of the protocol: (a) Proteins are incubated with the PulsIn lipid mixture transduction medium (see Materials and Methods); (b) following incubation, vesicles containing the proteins are formed; (c) C. elegans are incubated with the vesicles overnight; (d) proteins are absorbed in the gut and can diffuse to other tissues of the animals.