Figure 2.

Assessment of the transduction efficiency using a fluorescent protein. (a) Fluorescence and bright-field pictures of representative C. elegans worms at different incubation times from the treatment (treatment which consisted of a 2–12 hours incubation, see Materials and Methods) with vesicles loaded with the fluorescence marker mCherry (top series) or without vesicles (bottom series). All images were taken with a fluorescence microscopy setup at a magnification of 20X. (b) Bar plot showing the mCherry fluorescence signal integrated over the whole worm body at different incubation times from the treatment with the vesicles. (c) Confocal microscopy pictures of different part of the body of representative worms were taken at 10X magnification (left panels). Bright-field, fluorescence images and merged image of the two channels are also shown. High magnification images at 20X magnification are also shown (right panels). The error is given as the standard error on the mean (SEM). For quantification, ca. 50 worms were considered. Scale bars represent 80 μm and 40 μm in high magnification images (right panels).