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. 2017 Nov 8;7:15045. doi: 10.1038/s41598-017-13755-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Application of the transduction protocol for localisation studies using monoclonal antibodies. (a) Confocal microscopy pictures of a representative poly-Q:YFP C. elegans worm treated with vesicles loaded with an Alexa647-monoclonal antibody targeting the YFP. Different channels are shown: Bright-field, YFP fluorescence, Alexa647, merge of the fluorescence channels and overlay of all channels are shown. (b) Close-up image of the aggregates in panel b acquired at 20X magnification; same channels are shown. For colocalization images at low magnification (top panel) r = 0.21 and tM1 = 0.38 and tM2 = 0.54 in the low magnification images (top row)'. For images at high magnification r = 0.55 and tM1 = 0.37 and tM2 = 0.64 (bottom row). Scale bars represent 80 μm and 40 μm in the high magnification images.