Table 2.

Kinetic parameters of SyDdhs for pyruvate in the presence of 2.5 mM MgCl₂.

	k cat (s−1)	S 0.5 (mM)	k cat/S 0.5 (s−1 mM−1)	K i (mM)
SyDdh	3.53 ± 0.11	0.59 ± 0.05	6.00 ± 0.59	7.99 ± 0.93
SyDdh_Q14E	3.74 ± 0.22	0.14 ± 0.003	25.94 ± 1.09

Parameters were calculated as described in the Materials and Methods. k _cat, S _0.5, and k _cat/S _0.5 are the turnover rate, half-maximum concentration giving rise to 50% V _max, and catalytic efficiency, respectively. The inhibition constant (K _i) is shown when the data exhibited substrate inhibition. Data represent the mean ± SD from three independent experiments.