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. 2017 Nov 8;7:15042. doi: 10.1038/s41598-017-14886-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Production of recombinant full-length NS5A protein and domains I (D1), II (D2), and III (D3). Panel A shows schematic representations of the DNA constructs for production of recombinant full length 6× His-tagged-NS5A and glutathione S-transferase (GST)-tagged D1, D2 and D3 of the NS5A. Panel B shows purified recombinant NS5A and D1, D2, and D3. From left to right lanes: PageRuler™ Prestained Protein Ladder, purified 6× His-tagged-NS5A, GST protein, GST-tagged-D1, GST-tagged-D2, and GST-tagged-D3, respectively. Numbers at the left of Panel B are protein molecular masses in kDa.