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. 2017 Nov 8;7:15077. doi: 10.1038/s41598-017-14527-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative abundances of bacterial classes (A) and archaeal genera (B) represented in the v3–4 16S rDNA amplicons obtained for individual reactor samples. Each bar represents one sample, and is labelled as follows: D indicates the experimental day; HA1 and HA2 refer to the two reactors operated at 3.7 ± 0.2 g NH₄-N L⁻¹, and LA1, and LA2 refers to the reactors operated at 1.9 ± 0.2 g NH₄-N L⁻¹. OTUs that could not be classified at the domain level are labeled “Unclassified”, while OTUs that could not be classified at class or genus level for bacteria and archaea, respectively, are labeled *. Only taxa represented by a portion of ≥1% of the sequence reads in at least one of the samples are shown. “Others” include all reads representing the taxa with <1% abundance in all samples.