Table 2.

ASOs targeting (+)RNA5.

Name	Length (nt)	Sequence 5′ → 3′a	Complementary (+)RNA5 regionb	Conservation in target region nucleotide sequencec (%)
1430A	17	CGaAGAcUCCcCGcCCC	1427–1439	97.9
85A	11	CCaGUcUCcAU	82–92	94.0
150A	18	CUcCCGAUUcCACcAAUC	140–158	90.3
200A	13	CCaUCaUAAuCAC	194–206	89.6
285A	11	UGcUCuUCuAG	280–290	91.3
355A	16	UUcUCuCAUcCACuUU	352–370	88.0
415A	17	UUcGCCaUUGuUUGCUU	410–426	87.5
470A	13	CAuUCaGGUuGGA	466–478	93.0
600A	18	GUuCCaACUcCUUuCACC	591–608	87.6
615A	13	CUcCAuUGCuAUU	609–621	88.6
640A	17	CCaCGUuUGAuCAUUCU	628–644	86.7
760A	19	CCAuCAUuGCCcUCUgGGC	745–763	93.1
840A	12	CCcCUcAGAaUG	837–848	86.2
920A	13	CCuUCcCUUuCAA	917–929	87.2
1320A	21	GCUGCCAuAACGGUUGCUCUU	1308–1328	88.5
400A	11	CcAAAcUcUcC	398–405	88.6
727A	12	AuUuUCcUuUGA	722–732	91.6
950A	14	GAAuGGGuCUAuCC	943–957	90.6
2A	11	CAuUCuCAuUU	1146–1156	93.4
3A	16	CAuUCuCAuUUGAAGC	1141–1156	95.2
2B	11	UCcAGgGUaUU	1174–1184	88.2
Neg5*	11	CUGaACGCuUA	1262–1272	85.1
N*	17	AGACCUCUAUAGCAGCU	—	—

^aNucleotides in capital letter (A, C, G, U, D) are 2′-O-methyl-RNA nucleotides, lower case letters (a, c, g, u, d) are LNA nucleotides, ^bnumbering according to (+)RNA5 *-control ASO, Neg5 is complementary to double stranded region, N is not effective oligonucleotide used as control in published paper¹⁷. ^cCalculated for type A influenza virus strains based on sequences database used in this study.