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. 2017 Sep 19;14(6):6888–6894. doi: 10.3892/ol.2017.7000

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Figure 1. — EN2 gene promoter methylation status determined in primary kidney tissues and cell lines. (A) EN2 gene promoter methylation in primary cc-RCC tissues and corresponding non-malignant kidney tissues as indicated by MSP analysis. (B) EN2 gene promoter methylation in cc-RCC cell lines: 786-O, ACHN, A498 and normal human proximal tubule epithelial cell line HK-2 as indicated by MSP analysis. cc-RCC, clear cell renal cell carcinoma; EN2, engrailed-2; MSP, methylation-specific polymerase chain reaction; M, presence of methylated DNA; U, presence of unmethylated DNA; T, primary cc-RCC tissue, T1, tissue sample 1, T2, tissue sample 2; N, non-malignant kidney tissue; X, methylated positive control; Y, unmethylated positive control; Z, negative control.