Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 8;7:15008. doi: 10.1038/s41598-017-14932-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Caveolin-1 (Cav1) regulates cell spreading and focal adhesion (FA) assembly via modulation of protein levels of β1 integrin. (a) Representative western blot analysis results of NMuMG cells grown on matrices of varying stiffness for the indicated times. The protein levels of Cav1 were analyzed. Also see Supplementary Fig. S5. (b) Representative western blot analysis results of control vector (mock) - or Cav1-transfected NMuMG cells grown on matrices of varying stiffness for 4 h. The protein levels of β1 integrin and Cav1 were analyzed. Also see Supplementary Fig. S5. (c) Quantitative results of β1 integrin were from (b) and two other experiments. β-actin-normalized data in each condition was compared with those of cells grown on tissue culture plastic (>G Pa). (d) Representative confocal immunofluorescence images of mock- or Cav1-transfected NMuMG cells grown under indicated conditions for 4 h. Cells were stained for paxillin (green), F-actin (red), and nuclei (blue). (e) Quantification results show the average cell size as well as the size and number of FAs in cells grown on indicated conditions. At least 10 representative images from each condition were analyzed. All data are expressed as relative mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.