Figure 6.

Effect of miR-21 on the downregulation of PTEN induced by TGF-β1. (A) PTEN protein from MCF-7 cells was exposed with 5 ng/ml TGF-β1 alone or transfected with anti-miR-21 or scramble anti-miR-NC, and subsequently determined using western blot analysis and normalized to GAPDH. PTEN protein was significantly decreased in the presence of TGF-β1. (B) The relative fold changes were calculated. Following the downregulation of miR-21, using anti-miR-21, in MCF-7 cells, the repression of PTEN by TGF-β1 was rescued. Data are presented as the mean ± standard error of the mean of three independent assays. **P<0.01; ***P<0.001. miR, microRNA; PTEN, phosphatase and tensin homolog; TGF-β1, transforming growth factor β1; NC, negative control.